GLK-gel Affinity Chromatography Resin
Cross-linked Agarose Type
GLKgel Agarose media offer the highest specificity and selectivity in biomolecular separations and purifications. Purifications up to several orders of magnitude can be achieved in a single step. Affinity separation can often remove contaminants difficult to eliminate using conventional chromatographic procedures.
- Uniform particle size, High resolution, Low poriness
- High column efficiency
- Excellent bonding and end-capping technologies
Products
| Pr A 4FF | Pr G 4FF | IgM 6HP | IgY 6HP | |
| Substrate | 4% cross-linked agarose | 6% cross-linked agarose | ||
| Ligand | rProtein A | rProtein G | IgM | IgY |
| Particle Size | 90µm (45-165µm) | 37µm (25-45µm) | ||
| Capacity | 20mg hIgG/ml | 25mg hIgG/ml | 5mg hIgG/ml | 20mg hIgG/ml |
| pH Stability | 2-10 (Short) 3-9 (Long) | 2-13 (Short) 3-11 (Long) | ||
| Max. Pressure | 0.3MPa | |||
| Flow Rate | 300cm/h | 300cm/h | 150cm/h | 150cm/h |
| Storage | 4-8 °C, 20% EtOH | |||
Alkaline-resistant Agarose Protein A
| Items | Performance |
| Matrix | Highly cross-linked agarose particles |
| Average particle size | ~60µm |
| Ligand | Super alkaline resistant Protein A |
| Combined loading Capacity* | > 80 mg hlgG/ml media |
| Chemical stability | Common reagents used in the purification of tolerable antibodies |
| Working pH | 3-10 |
| Clean-in-Place | 0.1-0.5 M NaOH |
| Recommended linear flow rate | 100-500 cm/h |
| Save | 1XPBS with 20% ethanol, 2-8℃ |
*Note: The dynamic binding capacity of the target antibody needs to be determined by front-end analysis using the actual sample. The dynamic binding load is a function of the retention time of the sample and therefore needs to be defined over a range of retention times of the sample.
Application
Purification of IgG in human serum
- Sample: 5ml human serum with five times dilution (different buffers)
- Column: HT01 1.0ml Protein G 4FF
- Balance：A 0.02 M PB pH7.0; B 0.02M PB, 0. 3M NaCl pH 7.0
- Elution: 0.1 M Glycine-HCl pH2.7
- Flow Rate: 0.25m/min (sampling), 1ml/min
Protein Purification
Related Products
PS-DVB Substrate
- Hydrophobic PSDVB particles
- Super rigidity, low back pressure
- Excellent dynamic binding capacity
- Alkali resistance, 0.1-0.5M NaOH CIP
Ni Affinity Resin
- Ni NTA/NTA+ Resin
- Ni IDA Resin
- Ni TED Resin
- Benzamidine Affinity Media
mAb Analysis Column For HPLC
- Direct use in HPLC system
- About 3000-5000 circles (testing method)
- Fast and accurate analysis of antibody concentration
- Wide linearity range