Gel Filtration Chromatography Resin

Gel filtration chromatography achieves the purpose of separation according to the size of solute molecules. Gel filtration chromatography is also known as volume exclusion chromatography, molecular sieve chromatography. Gel filtration chromatography media are inert spherical granular materials with a porous network structure.

GALAK provides two types of gel filtration media: crosslinked agarose microspheres and crosslinked glucan microspheres.

Gel Filtration Chromatography

GLKgel GFC Agarose Media

GALAK has two types of agarose gel filtration media: 4% and 6% agarose gel. Crosslinked agarose gel (CL) is a low-strength crosslinking agarose gel with better physical and chemical stability. High-velocity agarose gel (FF) is high strength crosslinking agarose gel with good physical and chemical stability, can be sterilized by heat and humidity, and withstand various working conditions in protein production.

Products Molecular Weight (globulin) Particle Size Pressure Flow Rate pH Range
GFC 4B 6×104-2×107 90μm (45-165μm)

 

Customized Size:

25-46μm & 165-300μm

≤0.02MPa 12 cm/h 4-9 (Long) 4-9 (Short)
GFC 4FF 7×104-2×107 ≤0.3MPa 250 cm/h 2-12 (Long) 2-14 (Short)
GFC 6B 1×104-4×106 ≤0.02MPa 14 cm/h 4-9 (Long) 4-9 (Short)
GFC CL-6B 1×104-4×106 ≤0.05MPa 30 cm/h 3-12 (Long) 2-14 (Short)
GFC 6FF 1×104-4×106 ≤0.3MPa 300 cm/h 2-12 (Long) 2-14 (Short)

GLKgel GFC Glucan Media

Cross-linked glucan is a globular gel that contains a large number of hydroxyl groups and is prone to swelling in water and electrolyte solutions. G-type cross-linked glucans have different crosslinking degrees, their swelling degree and separation range are different. The swelling degree of crosslinked glucan is not affected by the presence of salt and detergent.

Products Molecular Weight (globulin) Particle Size Swelling Degree Flow Rate pH Range
GFC G-25 Coarse 1×103-5×103 165-300μm 4-6 ml/g 300 cm/h 2-13
GFC G-25 45-165μm 4-6 ml/g 150 cm/h
GFC G-75 3×103-8×104 45-165μm 12-15 ml/g 80 cm/h
GFC G-75 Fine 25-45μm 12-15 ml/g 20 cm/h

 

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