Gel Filtration Chromatography Resin
Gel filtration chromatography achieves the purpose of separation according to the size of solute molecules. Gel filtration chromatography is also known as volume exclusion chromatography, molecular sieve chromatography. Gel filtration chromatography media are inert spherical granular materials with a porous network structure.
GALAK provides two types of gel filtration media: crosslinked agarose microspheres and crosslinked glucan microspheres.
GLKgel GFC Agarose Media
GALAK has two types of agarose gel filtration media: 4% and 6% agarose gel. Crosslinked agarose gel (CL) is a low-strength crosslinking agarose gel with better physical and chemical stability. High-velocity agarose gel (FF) is high strength crosslinking agarose gel with good physical and chemical stability, can be sterilized by heat and humidity, and withstand various working conditions in protein production.
| Products | Molecular Weight (globulin) | Particle Size | Pressure | Flow Rate | pH Range |
| GFC 4B | 6×104-2×107 | 90μm (45-165μm)
Customized Size: 25-46μm & 165-300μm | ≤0.02MPa | 12 cm/h | 4-9 (Long) 4-9 (Short) |
| GFC 4FF | 7×104-2×107 | ≤0.3MPa | 250 cm/h | 2-12 (Long) 2-14 (Short) | |
| GFC 6B | 1×104-4×106 | ≤0.02MPa | 14 cm/h | 4-9 (Long) 4-9 (Short) | |
| GFC CL-6B | 1×104-4×106 | ≤0.05MPa | 30 cm/h | 3-12 (Long) 2-14 (Short) | |
| GFC 6FF | 1×104-4×106 | ≤0.3MPa | 300 cm/h | 2-12 (Long) 2-14 (Short) |
GLKgel GFC PG Media
GLK GFC PG series is gel filtration chromatography media (volume exclusion chromatography) for fine purification of biomolecules and buffer replacement on laboratory and industrial production scales.
| Products | GFC G-30PG | GFC G-75PG | GFC G-200PG |
| Particle Size | 35μm (25-45μm) | ||
| Separation Range | ≤ 10kDa (globulin) | 3-70kDa (globulin) | 10-600kDa (globulin) |
| pH Range | 3-12 (long), 1-14 (short) | ||
| Pressure Resistance | 0.3MPa | ||
| Flow Rate | 30-50cm/h, 90cm/h (max.) | ||
| Chemical Resistance | 8M urea, 6M Guanidine HCl, 70% EtOH, 1M NaOH | ||
| Autoclaving | 121℃×30min (0.5M NaCl, pH 7.0) | ||
| Storage | 0.2M NaAc, 20%EtOH, 4-30 ℃ | ||
