HILIC Column
Hydrophilic interaction liquid chromatography (HILIC) is a chromatographic technique used to improve the retention of strong polar substances in reversed-phase chromatography. It works by adopting a strong polar stationary phase and combining the mobile phase with a high proportion of organic phases. HILIC has a wide variety of stationary phases, and in principle, any stationary phase with the polar surface can use in HILIC mode. Therefore, stationary phases such as silica, amino (NH2), diol, and cyanogen (CN) in the positive phase chromatography can also use as stationary phases for HILIC.
Galaksil® HILIC-Diol
| Particle Size | Pore Size | Surface Area | Carbon Content | pH Range |
| 3/5/10um | 120Å | 330m2/g | 10% | 2-8 |
Galaksil® HILIC-Amide
| Particle Size | Pore Size | Surface Area | Carbon Content | pH Range |
| 3/5/10um | 120Å | 330m2/g | 8% | 2-8 |
Galaksil® HILIC-Imidazole
| Particle Size | Pore Size | Surface Area | Carbon Content | pH Range |
| 3/5/10um | 120Å | 330m2/g | 6% | 2-8 |
Application
Column: HILIC-Diol 5 μm
Dimension: 4.6×250mm
Mobile phase:
Blue: 90/10 v/v MeCN /100 mM HCOONH4 (pH3.6)
Black: 90/10 v/v MeCN /100 mM HCOONH4 (pH5.2)
Flow rate: 1 mL/min
Temperature: 30℃
Injection: 5 μL
Detection: 218 nm
Peaks: 1. DICY; 2. MET; 3. Melamine.
Column: HILIC-Amide 5 μm
Dimension: 4.6×250mm
Mobile phase:
80/10 v/v MeCN /100 mM CH3COOH
Flow rate: 0.5 mL/min
Temperature: 20℃
Injection: 10 μL
Detection: CDA
Peaks: 1. Stachydrine
Order Information
| Particle Size(µm) | Column I.D. (mm) | Length(mm) | Product Name | ||
| HILIC-Diol | HILIC-Amide | HILIC-Imidazole | |||
| 5 | 4.6 | 250 | A020-050012-04625 | A068-050012-04625 | A208-050012-04625 |
| 150 | A020-050012-04615 | A068-050012-04615 | A208-050012-04615 | ||
| 100 | A020-050012-04610 | A068-050012-04610 | A208-050012-04610 | ||
| 50 | A020-050012-04605 | A068-050012-04605 | A208-050012-04605 | ||
| 3 | 4.6 | 150 | A020-030012-04615 | A068-030012-04615 | A208-030012-04615 |
| 100 | A020-030012-04610 | A068-030012-04610 | A208-030012-04610 | ||
| 50 | A020-030012-04605 | A068-030012-04605 | A208-030012-04605 | ||
| 30 | A020-030012-04603 | A068-030012-04603 | A208-030012-04603 | ||
| 5 | 4.6 | 10 | A020-030012-04601 | A068-030012-04601 | A208-030012-04601 |
| 3 | 4.6 | 10 | A020-030012-04601 | A068-030012-04601 | A208-030012-04601 |
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