HILIC Column

Hydrophilic interaction liquid chromatography (HILIC) is a chromatographic technique used to improve the retention of strong polar substances in reversed-phase chromatography. It works by adopting a strong polar stationary phase and combining the mobile phase with a high proportion of organic phases. HILIC has a wide variety of stationary phases, and in principle, any stationary phase with the polar surface can use in HILIC mode. Therefore, stationary phases such as silica, amino (NH2), diol, and cyanogen (CN) in the positive phase chromatography can also use as stationary phases for HILIC.

Galaksil® HILIC-Diol

Particle Size Pore Size Surface Area Carbon Content pH Range
3/5/10um 120Å 330m2/g 10% 2-8

Galaksil® HILIC-Amide

Particle Size Pore Size Surface Area Carbon Content pH Range
3/5/10um 120Å 330m2/g 8% 2-8

Galaksil® HILIC-Imidazole

Particle Size

Pore Size Surface Area Carbon Content pH Range
3/5/10um 120Å 330m2/g 6%

2-8

Application

hilic diol column

Column: HILIC-Diol 5 μm

Dimension: 4.6×250mm

Mobile phase:

Blue: 90/10 v/v MeCN /100 mM HCOONH4 (pH3.6)

Black: 90/10 v/v MeCN /100 mM HCOONH4 (pH5.2)

Flow rate: 1 mL/min

Temperature: 30℃

Injection: 5 μL

Detection: 218 nm

Peaks: 1. DICY; 2. MET; 3. Melamine.

hilic amide column

Column: HILIC-Amide 5 μm

Dimension: 4.6×250mm

Mobile phase:

80/10 v/v MeCN /100 mM CH3COOH

Flow rate: 0.5 mL/min

Temperature: 20℃

Injection: 10 μL

Detection: CDA

Peaks: 1. Stachydrine

 

Order Information

Particle Size(µm) Column I.D. (mm) Length(mm) Product Name
HILIC-Diol HILIC-Amide HILIC-Imidazole
5 4.6 250 A020-050012-04625 A068-050012-04625 A208-050012-04625
150 A020-050012-04615 A068-050012-04615 A208-050012-04615
100 A020-050012-04610 A068-050012-04610 A208-050012-04610
50 A020-050012-04605 A068-050012-04605 A208-050012-04605
3 4.6 150 A020-030012-04615 A068-030012-04615 A208-030012-04615
100 A020-030012-04610 A068-030012-04610 A208-030012-04610
50 A020-030012-04605 A068-030012-04605 A208-030012-04605
30 A020-030012-04603 A068-030012-04603 A208-030012-04603
5 4.6 10 A020-030012-04601 A068-030012-04601 A208-030012-04601
3 4.6 10 A020-030012-04601 A068-030012-04601 A208-030012-04601

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