HPLC Column Selection By USP Specification
The following list of USP (United States Pharmacopoeia) column specifications (USP 35) includes a selection of recommended columns within each category. In most cases, there are several columns available within a given category, but in a few indicated instances a packing very closely fitting the specification has been included. Please contact us for further advice and assistance on selecting a suitable column by USP specification. Please also contact us for advice on column selection by EP (European Pharmacopoeia) specification. The USP monographs allow chromatographers flexibility to make method adjustments within specified limits in order to meet system suitability requirements.
| L1 | Octadecylsilane chemically bonded to porous or non-porous silica or ceramic microparticles, 1.5 to 10μm in diameter or a monolithic rod | GALAK C18 Column |
| L2 | Octadecylsilane chemically bonded to silica gel of a controlled surface porosity that has been bonded to a solid spherical core, 30 to 50μm in diameter | |
| L3 | Porous silica particles, 1.5 to 10μm in diameter, or a monolithic silica rod | GALAK Silica Column |
| L4 | Silica gel of controlled surface porosity bonded to a solid spherical core, 30 to 50μm in diameter | GALAK Silica Column |
| L5 | Alumina of controlled surface porosity bonded to a solid spherical core, 30 to 50μm in diameter | |
| L6 | Strong cation-exchange packing – sulphonated fluorocarbon polymer coated on a solid spherical core, 30 to 50μm in diameter | |
| L7 | Octylsilane chemically bonded to totally or superficially porous silica particles, 1.5 to 10μm in diameter, or a monolithic silica rod | GALAK C8 Column |
| L8 | An essentially monomolecular layer of aminopropylsilane chemically bonded to totally porous silica gel support, 1.5 to 10μm in diameter | GALAK NH2 Column |
| L9 | Irregular or spherical, totally porous silica gel having a chemically bonded, strongly acidic cation-exchange coating, 3 to 10μm in diameter | |
| L10 | Nitrile groups chemically bonded to porous silica particles, 1.5 to 10μm in diameter | GALAK CN Column |
| L11 | Phenyl groups chemically bonded to porous silica particles, 1.5 to 10μm in diameter | GALAK Phenyl Column |
| L12 | A strong anion-exchange packing made by chemically bonding a quaternary amine to a solid silica spherical core, 30 to 50μm in diameter | |
| L13 | Trimethylsilane chemically bonded to porous silica particles, 3 to 10μm in diameter | |
| L14 | Silica gel having a chemicallly bonded, strongly basic quaternary ammonium anion-exchange coating, 5 to 10μm in diameter | |
| L15 | Hexylsilane chemically bonded to totally porous silica particles, 3 to 10μm in diameter | |
| L16 | Dimethylsilane chemically bonded to porous silica particles, 5 to 10μm in diameter | |
| L17 | Strong cation-exchange resin consisting of sulphonated cross-linked styrene-divinylbenzene copolymer in the hydrogen form, 6 to 12μm in diameter | GALAK Sugar-10H Column |
| L18 | Amino and cyano groups chemically bonded to porous silica particles, 3 to 10μm in diameter | |
| L19 | Strong cation-exchange resin consisting of sulphonated cross-linked styrene-divinylbenzene copolymer in the calcium form, about 9μm in diameter | GALAK Sugar-10Ca Column |
| L20 | Dihydroxypropane groups chemically bonded to porous silica or hybrid particles, 1.5 to 10μm in diameter | GALAK HILIC-Diol Column |
| L21 | A rigid, spherical styrene-divinylbenzene copolymer, 3 to 30μm in diameter | |
| L22 | A cation-exchange resin made of porous polystyrene gel with sulphonic acid groups, about 10μm in size | |
| L23 | An anion-exchange resin made of porous polymethacrylate or polyacrylate gel with quaternary ammonium groups, 7 to 12μm in size | |
| L24 | Polyvinyl alcohol chemically bonded to porous silica particles, 5μm in diameter | |
| L25 | Packing having the capacity to separate compounds with a molecular weight range from 100-5000 (as determined by polyethylene oxide), applied to neutral, anionic, and cationic water-soluble polymers. A polymethacrylate resin base, cross-linked with polyhydroxylated ether (surface contained some residual carboxyl functional groups) was found suitable | |
| L26 | Butylsilane chemically bonded to totally porous silica particles, 1.5 to 10μm in diameter | GALAK C4 Column |
| L27 | Porous silica particles, 30 to 50μm in diameter | |
| L28 | A multifunctional support, which consists of a high purity, 100A, spherical silica substrate that has been bonded with anionic exchanger, amine functionality in addition to a conventional reversed-phase C8 functionality | |
| L29 | Gamma alumina, reversed-phase, low carbon percentage by weight, alumina-based polybutadiene spherical particles, 5μm in diameter with a pore volume of 80A units | |
| L30 | Ethylsilane chemically bonded to totally porous silica particles, 3 to 10μm in diameter | |
| L31 | A hydroxide-selective, strong anion-exchange resin-quaternary amine bonded on latex particles attached to a core of 8.5μm macroporous particles having a pore size of 2000A units and consisting of ethylvinylbenzene cross-linked with 55% divinylbenzene | |
| L32 | A chiral ligand-exchange resin packing - L-proline copper complex covalently bonded to irregularly shaped silica particles, 5 to 10μm in diameter | |
| L33 | Packing having the capacity to separate dextrans by molecular size over a range of 4,000 to 500,000 Da. It is spherical, silica-based, and processed to provide pH stability | |
| L34 | Strong cation-exchange resin consisting of sulphonated cross-linked styrene-divinylbenzene copolymer in the lead form, about 7 to 9μm in diameter | |
| L35 | A zirconium-stabilised spherical silica packing with a hydrophilic (diol-type) molecular monolayer bonded phase having a pore size of 150A | |
| L36 | A 3,5-dinitrobenzoyl derivative of L-phenylglycine covalently bonded to 5μm aminopropyl silica | |
| L37 | Packing having the capacity to separate proteins by molecular size over a range of 2,000 to 40,000 Da. It is a polymethacrylate gel | |
| L38 | A methacrylate-based size-exclusion packing for water-soluble samples | |
| L39 | A hydrophilic polyhydroxymethacrylate gel of totally porous spherical resin | |
| L40 | Cellulose tris-3,5-dimethylphenylcarbamate coated porous silica particles, 5μm to 20μm in diameter | GALAK Chrial OD |
| L41 | Immobilized α1-acid glycoprotein on spherical silica particles, 5μm in diameter | |
| L42 | Octylsilane and octadecylsilane groups chemically bonded to porous silica particles, 5μm in diameter | |
| L43 | Pentafluorophenyl groups chemically bonded to silica particles by a propyl spacer, 1.5 to 10μm in diameter | |
| L44 | Multifunctional support, which consists of high purity, 60A, spherical silica substrate that has been bonded with a cationic exchanger, sulphonic acid functionality in addition to a conventional reversed-phase C8 functionality | |
| L45 | Beta cyclodextrin, R,S-hydroxypropyl ether derivative, bonded to porous silica particles, 5 to 10μm in diameter | |
| L46 | Polystyrene/divinylbenzene substrate agglomerated with quaternary amine functionalized latex beads, about 9μm to 11μm in diameter | |
| L47 | High capacity anion-exchange microporous substrate, fully functionalized with a trimethylamine group, 8μm in diameter | |
| L48 | Sulphonated, cross-linked polystyrene with an outer layer of sub-micron, porous, anion-exchange microbeads, 5 to 15μm in diameter | |
| L49 | A reversed-phase packing made by coating a thin layer of polybutadiene onto spherical porous zirconia particles, 3 to 10μm in diameter | |
| L50 | Multi-function resin with reversed-phase retention and strong anion-exchange functionalities. The resin consists of ethylvinylbenzene, 55% cross-linked with divinylbenzene copolymer, 3 to 15μm in diameter, and a surface area of not less than 350 m2/g. The substrate is coated with quaternary ammonium functionalized latex particles consisting of styrene crosslinked with divinylbenzene | |
| L51 | Amylose tris-3,5-dimethylphenylcarbamate-coated, porous, spherical, silica particles, 5 to 10μm in diameter | GALAK Chrial AD |
| L52 | A strong cation-exchange resin made of porous silica with sulphopropyl groups, 5 to 10μm in diameter | |
| L53 | Weak cation-exchange resin consisting of ethylvinylbenzene, 55% cross-linked with divinylbenzene copolymer, 3 to 15μm diameter. The substrate is surface grafted with carboxylic acid and/or phosphoric acid functionalized monomers. Capacity not less than 500 μEq/column | |
| L54 | A size-exclusion medium made of covalent bonding of dextran to highly cross-linked porous agarose beads, about 13μm in diameter | |
| L55 | A strong cation-exchange resin made of porous silica coated with the polybutadiene-maleic acid copolymer, about 5μm in diameter | |
| L56 | Propyl silane chemically bonded to totally porous silica particles, 3 to 10μm in diameter | |
| L57 | A chiral-recognition protein, ovomucoid, chemically bonded to silica particles, about 5μm in diameter, with a pore size of 120A | |
| L58 | Strong cation-exchange resin consisting of sulphonated cross-linked styrene-divinylbenzene copolymer in the sodium form, about 6 to 30μm in diameter | GALAK Sugar-10Na Column |
| L59
| Packing for the size exclusion separations of proteins (separation by molecular weight) over the range of 5 to 7000 kDa. It is spherical (1.5 to 10μm), silica or hybrid packing with a hydrophilic coating | |
| L60 | Spherical, porous silica gel, 10μm or less in diameter, the surface of which has been covalently modified with alkyl amide groups and end-capped | |
| L61 | A hydroxide-selective, strong anion-exchange resin consisting of a highly cross-linked core of 13μm microporous particles having a pore size less than 10A units and consisting of ethylvinylbenzene cross-linked with 55% divinylbenzene with a latex coating composed of 85nm diameter microbeads bonded with alkanol quaternary ammonium ions (6%) | |
| L62 | C30 silane bonded phase on a fully porous spherical silica, 3 to 15μm in diameter. | |
| L63 | Glycopeptide teicoplanin linked through multiple covalent bonds to a 100A unit spherical silica | |
| L64 | Strongly basic anion-exchange resin consisting of 8% crosslinked styrene-divinylbenzene copolymer with a quaternary ammonium group in the chloride form, 45 to 180μm in diameter | |
| L65 | Strongly acidic cation-exchange resin consisting of 8% sulphonated crosslinked styrene-divinylbenzene copolymer with a sulphonic acid group in the hydrogen form, 63 to 250μm in diameter | |
| L66 | A crown ether coated on a 5μm particle size silica gel substrate. The active site is (S)-18-crown-6-ether | |
| L67 | Porous vinyl alcohol copolymer with a C18 alkyl group attached to the hydroxyl group of the polymer, 2 to 10μm in diameter | |
| L68 | Spherical, porous silica, 10μm or less in diameter, the surface of which has been covalently modified with alkyl amide groups and not end-capped | GALAK HILIC-Amide Column |
| L69 | Ethylvinylbenzene/divinylbenzene substrate agglomerated with quaternary amine functionalized 130nm latex beads, about 6.5μm in diameter | |
| L70 | Cellulose tris(phenyl carbamate) coated on 5μm silica | |
| L71 | A rigid, spherical polymethacrylate, 4 to 6μm in diameter | |
| L72 | (S)-phenylglycine and 3,5-dinitroaniline urea linkage covalently bonded to silica | |
| L73 | A rigid, spherical polydivinylbenzene particle, 5 to 10μm in diameter | |
| L74 | A strong anion-exchange resin consisting of a highly cross-linked core of 7μm macroporous particles having a 100A average pore size and consisting of ethylvinylbenzene cross-linked with 55% divinylbenzene and an anion-exchange layer grafted to the surface, which is functionalized with alkyl quaternary ammonium ions | |
| L75 | A chiral-recognition protein, bovine serum albumin (BSA), chemically bonded to silica particles, about 7μm in diameter, with a pore size of 300A | |
| L76 | Silica-based weak cation-exchange material, 5μm in diameter. The substrate is surface polymerized polybutadiene-maleic acid to provide carboxylic acid functionalities. Capacity not less than 29 μEq/column | |
| L77 | Weak cation-exchange resin consisting of ethylvinylbenzene, 55% cross-linked with divinylbenzene copolymer, 6 to 9μm diameter. The substrate is surface grafted with carboxylic acid functionalized groups. Capacity not less than 500 μEq/column (4mm x 25cm) | |
| L78 | A silane ligand that consists of both reversed-phase (an alkyl chain longer than C8) and anion-exchange (primary, secondary, tertiary or quaternary amino) functional groups chemically bonded to porous, non-porous or ceramic microparticles, 1.0 to 50μm in diameter or a monolithic rod | |
| L79 | A chiral-recognition protein, human serum albumin (HSA), chemically bonded to silica particles, about 5μm in diameter | |
| L80 | Cellulose tris(4-methylbenzoate)-coated, porous spherical silica particles, 5μm in diameter | GALAK Chrial OJ |
| L81 | A hydroxide-selective, strong anion-exchange resin consisting of a highly cross-linked core of 9μm porous particles having a pore size of 2000A and consisting of ethylvinylbenzene cross-linked with 55% divinylbenzene with a latex coating composed of 70nm diameter microbeads (6% cross-linked) bonded with alkanol quaternary ammonium ions | |
| L82 | Polyamine chemically bonded to cross-linked polyvinyl alcohol polymer, 4-5μm in diameter | |
| L83 | A hydroxide-selective, strong anion-exchange resin, quaternary amine bonded on latex particles attached to a core of 10.5μm microporous particles having a pore size of 10A and consisting of ethylvinylbenzene cross-linked with 55% divinylbenzene | |
| L84 | Weak cation-exchange resin consisting of ethylvinylbenzene, 55% cross-linked with divinylbenzene copolymer, 5μm diameter. The substrate is surface grafted with carboxylic acid-functionalized groups. Capacity not less than 8400 μEq/column (5mm x 25cm) |
USP and EP Allowable Adjustments
| Parameter | USP Allowable Adjustment * | EP Allowable Adjustment * |
| Column Length | ±70% | ±70% |
| Column i.d. | ±25% | ±25% |
| Particle Size | –50% | –50% |
| Column Temperature | ±10℃ | ±10℃ |
| Flow Rate | ±50% | ±50% |
| Eluent pH | ±0.2 units | ±0.2 units |
| Concentration of Buffer Salts | ±10% | ±10% |
| Solvent A: B Ratio | Minor ±30% relative, but±10% absolute | Minor ±30% relative or ±2% absolute, ≤±10% absolute for other |
| Injection Volume | Any reduction | Any reduction |
| Change of UV Detector Wavelength | 0, but ±3nm between detectors | No change |
* United States Pharmacopoeia 34 (2011) Section 621; European Pharmacopoeia 6.0 (2010) Section 2.2.4.6
Tian Jing
Manager & Engineer in GALAK Chromatography. Master of Chemical Engineering.
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