HPLC System Components
- Column Oven
The separation is performed inside the column; therefore, it can be said that the column is the heart of an LC system. The theory of chromatography column has not changed since Tswett’s time. However, there has been continuous improvement in column development. The recent columns are often prepared in a stainless steel housing, instead of glass columns used in Tswett’s experiment. The packing material generally used is silica or polymer gels compared to calcium carbonate used by Tswett.
The eluent used for LC varies from acidic to basic solvents. Most column housing is made of stainless steel since stainless is tolerant towards a large variety of solvents. However, for the analysis of some analytes such as biomolecules and ionic compounds, contact with metal is not desired, thus polyether ether ketone (PEEK) column housing is used instead.
The change in eluent detected by a detector is in the form of the electronic signal, and thus it is still not visible to our eyes. In older days, the pen (paper)-chart recorder was popularly used. Nowadays, a computer-based data processor (integrator) is more common. There are various types of data processors; examples include a simple system consisting of the in-built printer and a word processor, and a personal computer type consisting of a display monitor, keyboard, and printer. Also, there is software that is specifically designed for liquid chromatography systems. It provides not only data acquisition but features like peak-fitting, baseline correction, automatic concentration calculation, molecular weight determination, etc…
|Liquid Infusion System||Dual-piston reciprocating parallel pump, equipped with a binary high-pressure mixer|
|Flow Rate Range||0.001-10mL/min|
|Max Output Pressure||42 MPa|
|Qualitative Repeatability||RSD6≤0.1% (naphthalene/methanol standards)|
|Quantitative Repeatability||RSD6≤0.3% (naphthalene/methanol standards)|
|Pressure Fluctuation||0.1 MPa|
|Model||P100SP (40 ml)|
|Flow Rate Range||0.01-40 mL/ min|
|Prsssure Range||0-25 MPa|
|Flow Rate Accuracy||RSD≤0.1%|
|Model||P100HSP(50 mL); P100HP(120 mL)|
|Flow Rate Range||0.01-50mL/min; 0.01-120mL/min|
|Pressure Range||0-42 MPa|
|Flow Rate Accuracy||RSD 0.2%|
Manager & Engineer in GALAK Chromatography. Master of Chemical Engineering.
During my college study, I found liquid chromatography to be a profound subject. I know the painful struggle a novice needs to go through to get started. I share this article to help you solve your problems quickly.
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