Magnetic Beads for histidine-tagged protein
rProtein A/G & Ni NTA Magarose Beads
rProtein A/G & Ni NTA magnetic beads design for simple small-scale purification of histidine-tagged proteins. Magarose beads are suitable for purification of a single sample or multiple samples in parallel for example in screening experiments.
GALAK Magarose Beads can be used together with Eppendorf microcentrifuge tubes and a magnetic rack, for example, MagRack 6. GALAK Magarose Beads are easily separated from the liquid phase during the different steps of the purification protocol.
rProtein A/G Magnetic Beads
Protein A and Protein G Magarose Beads are affinity chromatography media with a high affinity for antibodies from various species. The media are designed for high capacity, which makes them useful for efficient small-scale purification/screening of monoclonal and polyclonal antibodies.
The products are based on recombinant protein A or protein G ligands. Protein A and Protein G Magarose Beads provide flexible purification allowing a wide range of sample volumes and easy scaling up by varying the bead quantity.
Main Parameters
| Substrate | Magnetic agarose microspheres |
| Ligands | Recombinant protein A/G |
| Combined ability (/ml magnetic beads) | >10mg hlgG |
| Particle size(μm) | 30-100 |
| Storage buffer | 1XPBS containing 20% ethanol |
| Volume | Suspend in protection solution, 20% content |
| Storage temperature | 2°C-8°C |
Ni NTA Magnetic Beads
Ni NTA Magarose Beads is an affinity chromatography medium with a high affinity for histidine-tagged proteins from various sources. The medium consists of magnetic beads based on an NTA ligand and immobilized with nickel ions.
Purification of histidine-tagged proteins on NTA media is a balance between capacity and purity, modulated by the concentration of imidazole in the sample and in the binding/wash buffer. To simplify the purification procedure two purification protocols are included in this instruction, one with a focus on high capacity and the other one with a focus on high purity.
Ni NTA Magarose Beads provides flexible purification allowing a wide range of sample volumes and easy scale-up by varying the bead quantity and is suitable for purification of multiple samples in parallel.
Main Parameters
| Substrate | Magnetic agarose microspheres |
| Combined ability (/ml magnetic beads) | >10mg 6XHis-tagged protein |
| Particle size | 30-100 μm |
| Storage buffer | 1XPBS containing 20% ethanol |
| Magnetic bead volumes | Suspend in protection solution, 20% content |
| Protective buffer | 20% EtOH 1XPBS |
| Storage temperature | 2°C-8°C |
