GLK-gel Ni Affinity Resin
GLK-gel Ni affinity resin is a nickel metal-chelating chromatography medium，which IDA/NTA/ted on high cross-linked agarose. GLK gel Ni Affinity Media have advantages of excellent stability, biocompatibility, solvent compatibility, large capacity, good selectivity, high-resolution natural generation, and low cost.
- Ni NTA/NTA+ Resin
- Ni IDA Resin
- Ni TED Resin
GLK-gel Ni NTA/NTA+ Resin
GLK gel Ni NTA/NTA+ media use Ni2+ to interact with amino acids (histidine, cysteine, tryptophan) on the side chain of the protein. It is suitable for the separation and purification of His tag proteins and biomolecules interacting with Ni2+.
| Substrate | 6% high cross-linked agarose |
| Particle Size | 90µm (45-165μm) GLK Ni NTA; 37μm(25-45μm) GLK Ni NTA+ |
| Binding Capacity | Approx. 40 mg His (tag protein)/ml media |
| pH Stability | 3-12 (Working); 2-14 (Cleaning) |
| Max. Pressure | 300 bar (43.5 psi) |
| Chemical Stability | 0.01M HCl; 0.01M NaOH (one week);1M NaOH; 70%EtOH(12 hours); 2% SDS(1 hour); 30% isopropanol (0.5 hour) |
| Storage | 4-15 °C, 20℃ EtOH |
GLK-gel Ni IDA Resin
| Substrate | 6% high cross-linked agarose |
| Particle Size | 90µm (45-165μm) |
| Binding Capacity | Approx. 45 mg His (tag protein)/ml media |
| pH Stability | 3-12 (Working); 2-14 (Cleaning) |
| Max. Pressure | 300 bar (43.5 psi) |
| Chemical Stability | Common aqueous solutions and buffers. Avoid chelating agents (EDTA, EGTA) and reducing agents (DTT, DTE) |
| Storage | 4-15 °C, 20℃ EtOH |
GLK-gel Ni TED Resin
| Substrate | 6% high cross-linked agarose |
| Particle Size | 90µm (45-165μm) |
| Binding Capacity | Approx. 20 mg His (tag protein)/ml media |
| pH Stability | 3-12 (Working); 2-14 (Cleaning) |
| Max. Pressure | 300 bar (43.5 psi) |
| Chemical Stability | 0.01M hydrochloric acid; 0.01M sodium hydroxide (one week); 20mM EDTA; 10mM DTT; 1M sodium hydroxide; 8M urea; 100mM EDTA; 0.5m imidazole (2 hours); 30% isopropanol (20 min) |
| Storage | 4-15 °C, 20℃ EtOH |
Ni Affinity Resin Application
Ni NTA Resin
- E. coli cracking supernatant (His tag protein)
Ni IDA Resin
- E. coli cracking supernatant (His tag protein)
Related Product
Agarose Substrate
- High cross-linked agarose
- Good water-swellable
- Super biological compatibility
- Alkali resistance, 0.1-0.5M NaOH CIP
PS-DVB Substrate
- Hydrophobic PSDVB particles
- Super rigidity, low back pressure
- Excellent dynamic binding capacity
- Alkali resistance, 0.1-0.5M NaOH CIP
mAb Analysis Column For HPLC
- Direct use in HPLC system
- About 3000-5000 circles (testing method)
- Fast and accurate analysis of antibody concentration
- Wide linearity range