GLK-gel Ni Affinity Resin
GLK-gel Ni affinity resin is a nickel metal-chelating chromatography medium,which IDA/NTA/ted on high cross-linked agarose. GLK gel Ni Affinity Media have advantages of excellent stability, biocompatibility, solvent compatibility, large capacity, good selectivity, high-resolution natural generation, and low cost.
- Ni IDA Resin
- Ni TED Resin
GLK-gel Ni IDA Resin
Substrate | 6% high cross-linked agarose |
Particle Size | 90µm (45-165μm) |
Binding Capacity | Approx. 45 mg His (tag protein)/ml media |
pH Stability | 3-12 (Working); 2-14 (Cleaning) |
Max. Pressure | 300 bar (43.5 psi) |
Chemical Stability | Common aqueous solutions and buffers. Avoid chelating agents (EDTA, EGTA) and reducing agents (DTT, DTE) |
Storage | 4-15 °C, 20℃ EtOH |
GLK-gel Ni TED Resin
Substrate | 6% high cross-linked agarose |
Particle Size | 90µm (45-165μm) |
Binding Capacity | Approx. 20 mg His (tag protein)/ml media |
pH Stability | 3-12 (Working); 2-14 (Cleaning) |
Max. Pressure | 300 bar (43.5 psi) |
Chemical Stability | 0.01M hydrochloric acid; 0.01M sodium hydroxide (one week); 20mM EDTA; 10mM DTT; 1M sodium hydroxide; 8M urea; 100mM EDTA; 0.5m imidazole (2 hours); 30% isopropanol (20 min) |
Storage | 4-15 °C, 20℃ EtOH |
Ni Affinity Resin Application
Ni NTA Resin
- E. coli cracking supernatant (His tag protein)
Ni IDA Resin
- E. coli cracking supernatant (His tag protein)