GLK-gel Ni Affinity Resin

GLK-gel Ni affinity resin is a nickel metal-chelating chromatography medium,which IDA/NTA/ted on high cross-linked agarose. GLK gel Ni Affinity Media have advantages of excellent stability, biocompatibility, solvent compatibility, large capacity, good selectivity, high-resolution natural generation, and low cost.

  • Ni IDA Resin
  • Ni TED Resin
Nickel affinity resin

GLK-gel Ni IDA Resin

Substrate 6% high cross-linked agarose
Particle Size 90µm (45-165μm)
Binding Capacity Approx. 45 mg His (tag protein)/ml media
pH Stability 3-12 (Working); 2-14 (Cleaning)
Max. Pressure 300 bar (43.5 psi)
Chemical Stability Common aqueous solutions and buffers. Avoid chelating agents (EDTA, EGTA) and reducing agents (DTT, DTE)
Storage 4-15 °C, 20℃ EtOH

GLK-gel Ni TED Resin

Substrate 6% high cross-linked agarose
Particle Size 90µm (45-165μm)
Binding Capacity Approx. 20 mg His (tag protein)/ml media
pH Stability 3-12 (Working); 2-14 (Cleaning)
Max. Pressure 300 bar (43.5 psi)
Chemical Stability 0.01M hydrochloric acid; 0.01M sodium hydroxide (one week); 20mM EDTA; 10mM DTT; 1M sodium hydroxide; 8M urea; 100mM EDTA; 0.5m imidazole (2 hours); 30% isopropanol (20 min)
Storage 4-15 °C, 20℃ EtOH

Ni Affinity Resin Application

Ni NTA Resin

  • E. coli cracking supernatant (His tag protein)
Nickel affinity media

Ni IDA Resin

  • E. coli cracking supernatant (His tag protein)
Nickel affinity media

Related Product

Agarose Substrate

  • High cross-linked agarose
  • Good water-swellable
  • Super biological compatibility
  • Alkali resistance, 0.1-0.5M NaOH CIP

PS-DVB Substrate

  • Hydrophobic PSDVB particles
  • Super rigidity, low back pressure
  • Excellent dynamic binding capacity
  • Alkali resistance, 0.1-0.5M NaOH CIP

mAb Analysis Column For HPLC

  • Direct use in HPLC system
  • About 3000-5000 circles (testing method)
  • Fast and accurate analysis of antibody concentration
  • Wide linearity range

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