GLK-gel Ni Affinity Resin

GLK-gel Ni affinity resin is a nickel metal-chelating chromatography medium,which IDA/NTA/ted on high cross-linked agarose. GLK gel Ni Affinity Media have advantages of excellent stability, biocompatibility, solvent compatibility, large capacity, good selectivity, high-resolution natural generation, and low cost.

  • Ni NTA/NTA+ Resin
  • Ni IDA Resin
  • Ni TED Resin
Nickel affinity resin

GLK-gel Ni NTA/NTA+ Resin

GLK gel Ni NTA/NTA+ media use Ni2+ to interact with amino acids (histidine, cysteine, tryptophan) on the side chain of the protein. It is suitable for the separation and purification of His tag proteins and biomolecules interacting with Ni2+.

Substrate 6% high cross-linked agarose
Particle Size 90µm (45-165μm) GLK Ni NTA; 37μm(25-45μm) GLK Ni NTA+
Binding Capacity Approx. 40 mg His (tag protein)/ml media
pH Stability 3-12 (Working); 2-14 (Cleaning)
Max. Pressure 300 bar (43.5 psi)
Chemical Stability 0.01M HCl; 0.01M NaOH (one week);1M NaOH; 70%EtOH(12 hours); 2% SDS(1 hour); 30% isopropanol (0.5 hour)
Storage 4-15 °C, 20℃ EtOH

GLK-gel Ni IDA Resin

Substrate 6% high cross-linked agarose
Particle Size 90µm (45-165μm)
Binding Capacity Approx. 45 mg His (tag protein)/ml media
pH Stability 3-12 (Working); 2-14 (Cleaning)
Max. Pressure 300 bar (43.5 psi)
Chemical Stability Common aqueous solutions and buffers. Avoid chelating agents (EDTA, EGTA) and reducing agents (DTT, DTE)
Storage 4-15 °C, 20℃ EtOH

GLK-gel Ni TED Resin

Substrate 6% high cross-linked agarose
Particle Size 90µm (45-165μm)
Binding Capacity Approx. 20 mg His (tag protein)/ml media
pH Stability 3-12 (Working); 2-14 (Cleaning)
Max. Pressure 300 bar (43.5 psi)
Chemical Stability 0.01M hydrochloric acid; 0.01M sodium hydroxide (one week); 20mM EDTA; 10mM DTT; 1M sodium hydroxide; 8M urea; 100mM EDTA; 0.5m imidazole (2 hours); 30% isopropanol (20 min)
Storage 4-15 °C, 20℃ EtOH

Ni Affinity Resin Application

Ni NTA Resin

  • E. coli cracking supernatant (His tag protein)
Nickel affinity media

Ni IDA Resin

  • E. coli cracking supernatant (His tag protein)
Nickel affinity media

Related Product

Agarose Substrate

  • High cross-linked agarose
  • Good water-swellable
  • Super biological compatibility
  • Alkali resistance, 0.1-0.5M NaOH CIP

PS-DVB Substrate

  • Hydrophobic PSDVB particles
  • Super rigidity, low back pressure
  • Excellent dynamic binding capacity
  • Alkali resistance, 0.1-0.5M NaOH CIP

mAb Analysis Column For HPLC

  • Direct use in HPLC system
  • About 3000-5000 circles (testing method)
  • Fast and accurate analysis of antibody concentration
  • Wide linearity range

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