GLK-gel Ni Affinity Resin
GLK-gel Ni affinity resin is a nickel metal-chelating chromatography medium，which IDA/NTA/ted on high cross-linked agarose. GLK gel Ni Affinity Media have advantages of excellent stability, biocompatibility, solvent compatibility, large capacity, good selectivity, high-resolution natural generation, and low cost.
- Ni IDA Resin
- Ni TED Resin
GLK-gel Ni IDA Resin
| Substrate | 6% high cross-linked agarose |
| Particle Size | 90µm (45-165μm) |
| Binding Capacity | Approx. 45 mg His (tag protein)/ml media |
| pH Stability | 3-12 (Working); 2-14 (Cleaning) |
| Max. Pressure | 300 bar (43.5 psi) |
| Chemical Stability | Common aqueous solutions and buffers. Avoid chelating agents (EDTA, EGTA) and reducing agents (DTT, DTE) |
| Storage | 4-15 °C, 20℃ EtOH |
GLK-gel Ni TED Resin
| Substrate | 6% high cross-linked agarose |
| Particle Size | 90µm (45-165μm) |
| Binding Capacity | Approx. 20 mg His (tag protein)/ml media |
| pH Stability | 3-12 (Working); 2-14 (Cleaning) |
| Max. Pressure | 300 bar (43.5 psi) |
| Chemical Stability | 0.01M hydrochloric acid; 0.01M sodium hydroxide (one week); 20mM EDTA; 10mM DTT; 1M sodium hydroxide; 8M urea; 100mM EDTA; 0.5m imidazole (2 hours); 30% isopropanol (20 min) |
| Storage | 4-15 °C, 20℃ EtOH |
Ni Affinity Resin Application
Ni NTA Resin
- E. coli cracking supernatant (His tag protein)
Ni IDA Resin
- E. coli cracking supernatant (His tag protein)
Related Product
Agarose Substrate
- High cross-linked agarose
- Good water-swellable
- Super biological compatibility
- Alkali resistance, 0.1-0.5M NaOH CIP
PS-DVB Substrate
- Hydrophobic PSDVB particles
- Super rigidity, low back pressure
- Excellent dynamic binding capacity
- Alkali resistance, 0.1-0.5M NaOH CIP
mAb Analysis Column For HPLC
- Direct use in HPLC system
- About 3000-5000 circles (testing method)
- Fast and accurate analysis of antibody concentration
- Wide linearity range