Ni NTA Magnetic Beads
Ni NTA magnetic beads design for simple small-scale purification of histidine-tagged proteins. Magarose beads are suitable for purification of a single sample or multiple samples in parallel for example in screening experiments.
GALAK Magarose Beads can be used together with Eppendorf microcentrifuge tubes and a magnetic rack, for example, MagRack 6. GALAK Magarose Beads are easily separated from the liquid phase during the different steps of the purification protocol.
Ni NTA Magnetic Beads Parameters
| Substrate | Magnetic agarose microspheres |
| Combined ability (/ml magnetic taste) | >10mg 6XHis-tagged protein |
| Particle size | 30-100 μm |
| Storage buffer | 1XPBS containing 20% ethanol |
| Volume | Suspend in protection solution, 20% content |
| Protective buffer | 20% EtOH 1XPBS |
| Storage temperature | 2°C-8°C |
Ni NTA Magnetic Beads Introduction
Ni NTA Magarose Beads is designed for efficient/protein purification histidine labels quickly and design of new products, magnetic microspheres can step in under the action of the magnetic field directly from biological samples purify high purity of target protein, greatly simplifies the purification technology and purification efficiency, suitable for scientific research and industrial customers high flux to histidine label protein purification.
Compared with the traditional column chromatography method, Ni NTA Magarose Beads do not need to control the flow rate of sample preparation, or expensive chromatography and centrifugation equipment. The combination of samples and magnetic Beads and the separation of target proteins and magnetic Beads become very simple and fast, and it is easier to achieve a high-throughput, automated protein purification method.
Ni NTA Magarose Beads are based on 4% agarose gel as a matrix. Through the chemical coupling of tetrachonitric triacetic acid (NTA) and chelating nickel ions (N2+), form a very stable octahedral structure. Nickel ions are in the center of the octahedron, and nickel ions are more stable.
Reagent Tolerance of Ni NTA Magarose Beads
| Reagents | Concentration |
| Reducing agent | 5 mM DTE
1mM DTT 20 mM β-mercaptoethanol 5 mM TCEP 10mM reduced glutathione |
| Denaturing reagent | 8 M urea
6 M Gua-HCI |
| Abluent | 2% TritonTM X-100 (nonionic)
2% TweenTM 20 (nonionic) 2% NP-40 (nonionic) 2% cholate (anionic) 1% CHAPS (zwitterionic) |
| Other reagents | 500 mM imidazole
20% ethanol 50% glycerol 100 mM Na2SO4 1.5 M NaCl 1 mM EDTA 60 mM citrate |
| Buffer | 50 mM sodium phosphate, pH 7.4
100 mM Tris-HCl, pH 7.4 100 mM Tris-acetate, pH7.4 100 mM HEPES, pH 7.4 100 mM MOPS, pH 7.4100 mM sodium acetate pH 4 |
