SEC Column
GALAK SEC is a family of high-performance, size exclusion chromatography (SEC) columns for separating a broad range of biomolecules based on the size of the analyte. The column technology involves the creation of a neutral hydrophilic layer on the surface of specially designed high-strength, monodispersed, silica particles followed by a well-established production process. Therefore, GALAK SEC columns have abroad application range in pharmaceutical, biopharmaceutical, and academic research.
- High column efficiency, high resolution;
- Minimal undesired interactions between the stationary phase and analytes, resulting in good peak shape and recovery;
- High physical strength for better column lifetime;
- The broad range of applications, including small molecule drugs, peptides, proteins, oligos, glycans, etc.
Main Characters
- SEC-150 - designed for separating peptides, glycans, small oligos, small proteins.
- SEC-300 - designed for mAb aggregate determination.
- SEC-500 - designed for separating larger proteins and oligos.
| SEC-150 | SEC-300 | SEC-500 | |
| Ligand | Diol | ||
| Substrate | Monodisperse High-pure Silica Particle | ||
| Particle Size | 5um | ||
| Pore Size | 150A | 300A | 500A |
| pH Range | 2-8 | ||
| Temperature | <40℃ | ||
| Pressure | 6000psi | ||
| Ligand Range (PEG) | 200-15,000 | 1.000-100,000 | 5,000-200,000 |
| Ligand Range (Glucan) | 1,000-50,000 | 5,000-150,000 | 20,000-500,000 |
| Ligand Range (Globular Protein) | 5,000-150,000 | 10,000-1,000,000 | 20,000-2,000,000 |
Application
Column Black: SEC-150, 5μm
Column Blue: SEC-300, 5μm
Column Red: SEC-500, 5μm
Dimension: 4.6×300mm
Mobile phase: 150 mM Phosphate Buffered Saline (pH 6.8)
Flow rate: 0.35 mL/min
Temperature: 30 ℃
Injection: 5μL
Detection: UV 280 nm
Peaks:
1. Thyroglobulin (0.5mg/mL) -669,000Da
2. Conalbumin (1mg/mL) -75,000Da
3. Ribonuclease A (1mg/mL) -13,700Da
4. Uracil (0.1mg/mL) ‒112Da
Related Products
Affinity Resin
Reversed-phase Resin
