Chromatography Purification of Inactive Avian Influenza Virus
Vian influenza viruses (AIVs) are normally found only in birds, they can also cause infections among people. Globally, avian influenza viruses (AIVs) have been identified in more than 100 different wild bird species. The majority of the wild birds from which these viruses have been recovered represent gulls, terns, and shorebirds or waterfowl such as ducks, geese, and swans. These wild birds are often viewed as reservoirs (hosts) for avian influenza A viruses. ATVs can also sicken and kill domesticated birds, such as chickens, ducks, and turkeys.
Vian influenza vaccine is a good and effective way to protect fowl from AIVs. More and more farms inject ATV vaccine for their fowl to avoid the large area of infection that will cost a great loss. Of course, vaccine production is a very complicated process with various methods. Some of the vaccines are inactive viruses. In this test, we use the liquid chromatography method to purify the inactive AIVs.
Test Method
- Column: φ10×13 mm (1 mL VirCap® Inert Shell)
- Flow rate: 76 cm/h
- Balance buffer A: 50 mM Tris-HCl buffer, pH 8.0 Elution B: 1 M NaCl + 50 mM Tris-HCl buffer, pH 8.0
- Sample: Treat the avian influenza virus in 3000 rpm centrifugation for 10min. Then sampling after 0.45 μm membrane filtration
The picture below is the chromatograms of the tests of the avian influenza virus sample.
The left picture is the purification chromatogram of the original clear avian influenza virus sample with VirCap® InertShell (Red) and Competitor 700 (Black). After the collection of breakthrough peak 1, peak 2 (peak 1’, peak 2’), and elution peak 3 (peak 3’), analysis the collected fluid in Superdex 200 prepacked columns.
- VirCap® InertShell: the AIV target virus in breakthrough peak 1 has been purified and collected. No AIV target virus has been found in elution
- Competitor 700: there is a little AIV target virus in breakthrough peak 1’ and peak 2’. a little AIV target virus has appeared in elution
* Test results will change according to different instruments and operators.
