GLK-gel Affinity Chromatography Resin

Cross-linked Agarose Type

GLKgel Agarose media offer the highest specificity and selectivity in biomolecular separations and purifications. Purifications up to several orders of magnitude can be achieved in a single step. Affinity separation can often remove contaminants difficult to eliminate using conventional chromatographic procedures.

Uniform particle size, High resolution, Low poriness
High column efficiency
Excellent bonding and end-capping technologies

Products

	Pr A 4FF	Pr G 4FF	IgM 6HP	IgY 6HP
Substrate	4% cross-linked agarose		6% cross-linked agarose
Ligand	rProtein A	rProtein G	IgM	IgY
Particle Size	90µm (45-165µm)		37µm (25-45µm)
Capacity	20mg hIgG/ml	25mg hIgG/ml	5mg hIgG/ml	20mg hIgG/ml
pH Stability	2-10 (Short) 3-9 (Long)		2-13 (Short) 3-11 (Long)
Max. Pressure	0.3MPa
Flow Rate	300cm/h	300cm/h	150cm/h	150cm/h
Storage	4-8 °C, 20% EtOH

Alkaline-resistant Agarose Protein A

Items	Performance
Matrix	Highly cross-linked agarose particles
Average particle size	~60µm
Ligand	Super alkaline resistant Protein A
Combined loading Capacity*	> 80 mg hlgG/ml media
Chemical stability	Common reagents used in the purification of tolerable antibodies
Working pH	3-10
Clean-in-Place	0.1-0.5 M NaOH
Recommended linear flow rate	100-500 cm/h
Save	1XPBS with 20% ethanol, 2-8℃

*Note: The dynamic binding capacity of the target antibody needs to be determined by front-end analysis using the actual sample. The dynamic binding load is a function of the retention time of the sample and therefore needs to be defined over a range of retention times of the sample.