mAb Analysis HPLC Column
Protein A Affinity Column Absolut A
mAb analysis HPLC column is used for monoclonal antibody analysis in labs. The determination of IgG concentration (titer) uses for the rapid screening of cell culture samples and optimization of fermentation conditions. Protein A affinity chromatography is a fast, accurate, and reliable method. Absolut A is protein A affinity columns for mab analysis.
- Direct use on HPLC instrument
- Fast and accurate analysis of antibody concentration
- Wide linearity range
Comparison test report with P*R*S A column in China TOP CXO manufacturer, click here >>>
mAb Analysis HPLC Column Technical Parameters
| Ligand | Substrate | Pressure | pH Range | Dynamic Capacity | Flow Rate | Life Time | |
| Absolut A | rProtein A | 30 μm PSDVB | 1200psi | 2-11 | 40mg human IgG/ml media | 1-3ml/min | 3000 circles |
| Absolut A+ | rProtein A | 30 μm PSDVB | 1200psi | 2-11 | 40mg human IgG/ml media | 1-3ml/min | 5000 circles |
- Lifetime is based on laboratory tests. Actual data is according to sample properties.
- PS/DVB: Poly(styrene/divinylbenzene)
Advantages of Absolut A
- Mono-dispersed PS/DVB particles feature very high rigidity, low back pressure, and quick mass transfer.
- Unique hydrophilic surface treatment technology minimizes the nonspecific absorption and guarantees accurate determination of antibody concentration.
- The protein binds specifically to the Fc region of IgG, and the high selectivity guarantees the accuracy of concentration determination.
- Fast analysis cycle time: 2 – 5 minutes.
- Satisfactory linearity within a wide concentration range from 0.02 to 10 mg/ml.
- Long lifetime: 3000-5000 analysis cycles.
- New alkali-resistant Protein A ligand warrants 0.1-0.5M NaOH cleaning conditions.
Performance Testing For mAb Analysis HPLC Column
1. Excellent Linearity
2.Flexible choice of flow rate
3.Lifetime testing
Application
(1) Fc Fusion Protein
(2) Monoclonal antibody
Analysis Condition Of mAb Analysis HPLC Column
| Eluent A | 20-50 mM Phosphate, 150 mM NaCl, pH7-7.5 |
|---|---|
| Eluent B | 12 mM HCl, 150 mM NaCl, pH1.9, or 100 mM Gly-HCl, 150 mM NaCl, pH2.5 |
| Flow rate | 1 mL/min |
| Injection | 1-100 μL |
| Detection | UV 280nm |
Method Timetable
| Time(min) | Gradient(%B) | |
|---|---|---|
| 0.00 | 0 | Equilibration/Wash, 1 min, 10 CV |
| 1.00 | 0 | Equilibration/Wash, 1 min, 10 CV |
| 1.01 | 100 | Elution, 2 min, 20 CV |
| 3.00 | 100 | Elution, 2 min, 20 CV |
| 3.01 | 0 | Reequilibration, 2 min, 20 CV |
| 5.00 | 0 | Reequilibration, 2 min, 20 CV |
* the analysis condition varies according to different machines.
Order Information
| Product Name | Column Size | Column Tubing | Order Number |
| Absolut A | 2.1*30mm | Stainless Steel | 1107-030-2-02103 |
| 2.1*30mm | PEEK | 1107-030-1-02103 | |
| 4.6*50mm | Stainless Steel | 1107-030-2-04605 | |
| 4.6*50mm | PEEK | 1107-030-1-04605 | |
| Absolut A PLUS | 2.1*30mm | Stainless Steel | 1107-020-2-02103 |
| 2.1*30mm | PEEK | 1107-020-1-02103 | |
| 4.6*50mm | Stainless Steel | 1107-020-2-04605 | |
| 4.6*50mm | PEEK | 1107-020-1-04605 |
Related Product
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- Hydrophobic PS-DVB particles
- Super rigidity, low back pressure
- Excellent dynamic binding capacity
- Alkali resistance, 0.1-0.5M NaOH CIP
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- High cross-linked agarose substract
- Good water-swellable
- Super biological compatibility
- Alkali resistance, 0.1-0.5M NaOH CIP
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