Protein A Affinity Resin

Sepromax® A50

Sepromax® A50 is designed to analyze and purify monoclonal antibodies (mAbs). Compared to traditional agarose media Sepromax® A50 has the advantages of high dynamic binding capacity (DBC), long service life, and less shedding of ligand. NaOH (0.1-0.5M) can be used for clean-in-Place (CIP).
The ligand of Sepromax® A50 is recombinant protein A (rProtein A) immobilized on the surface of the macro-porous PS/DVB microsphere substrate. The rProtein A has a better alkali-resisting ability that ensures stability in high pH conditions. With our hydrophilic treatment and binding technology, we eliminated non-specific binding PS-DVB surface. Hence, Sepromax® A50 is extremely useful for the purification process of monoclonal antibodies.


  • High rigidity monodisperse matrix, low back pressure, suitable for large-scale downstream processing
  • Outstanding high dynamic binding capacity at low residence time reduces process time and the amount of medium used
  • Novel Protein A ligand allows the use of 0.1-0.5 M NaOH for CIP
  • Low ligand leakage ensures a long lifetime
  • High price-performance ratio

Main Characteristics

Support Matrix Poly(styrene/divinylbenzene) (PS-DVB)
Ligand Recombinant Protein A
Ave. Particle size 50µm
Dynamic Binding Capacity (DBC) Approx. 40 mg human IgG/ml media (Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 5 cm, Residence time 0.6 min)
Shrinkage/Swelling < 1% from 1-100% organic solvent
pH range (Long term) pH 2-10
Maximum Operating Pressure 1500 psi (100 bar / 10 MPa)
Cleaning Agents 0.1-0.5M NaOH
Temperature Stability 4-40 °C
Delivery Conditions 20% ethanol (2-8℃)

Performance Testing

1. Particle images

Protein A Affinity Resin sepromax A40
sepromax A40 image

2. Dynamic Binding Capacity (DBC)

Test Condition
System AKTA® Purifier10
Initial Buffer 20 mM PB,0.15 M NaCl,pH 7.4
Elution Buffer 0.1 M Gly-HCl, pH 2.5
CIP solution 0.5 M NaOH
Sample 1.0 mg/ml hIgG
GALAK Sepromax A50 Resin

3. Protein A Ligand Leakage Test (ELISA)

Samples: 20mg hlgG/ml-resin, 9mg mAb/ml-resin

ELISA test: Cygnus F400 kit

GALAK Sepromax A50 Resin
ELISA test for Protein A Affinity Resin sepromax A40

4. Alkali Resistance Test (150 Cycle Lifetime)

GALAK Sepromax A50 alkali resistance

Protein A Affinity Resin Application

1.Fc Protein

A40+-Fc-protein (1)

2.Monoclonal Antibody

A40+单抗纯化 (1)

Related Products

Agarose Substrate

  • High cross-linked agarose
  • Good water-swellable
  • Super biological compatibility
  • Alkali resistance, 0.1-0.5M NaOH CIP

Ni Affinity Resin

  • Ni NTA/NTA+ Resin
  • Ni IDA Resin
  • Ni TED Resin
  • Benzamidine Affinity Media
Protein A affinity Chromatography HPLC Column mab antibody analysis

mAb Analysis Column For HPLC

  • Direct use in HPLC system
  • About 3000-5000 circles (testing method)
  • Fast and accurate analysis of antibody concentration
  • Wide linearity range

There are plenty of manufacturers in chromatography resin market. But, we are the

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