Rosmarinic Acid Purification

GALAK present invention relates to the purification process of Rosmarinic acid. Its feature is first to dissolve the crude product containing Rosmarinic acid with alkaline buffer, and filtering obtains sample solution；Sample solution is loaded on boric acid affinity column again, first boric acid affinity column is rinsed with alkaline buffer, then eluted to boric acid affinity column with acidic buffer, obtains affinity elution liquid；Then, affinity elution liquid is loaded onto macroporous resin column, first rinses macroporous resin column with purified water, then eluted with ethanol-water, obtain ethanol-water elution liquid；Finally, being dried under reduced pressure to ethanol-water elution liquid, the Rosmarinic acid product purified. Rosmarinic acid is isolated and purified using this method, the preparation process is simple, and without using poisonous and hazardous organic solvent, purification efficiency is high, and product purity is high, is suitable for industrialized production.